منابع مشابه
A web server for performing electronic PCR
'Electronic PCR' (e-PCR) refers to a computational procedure that is used to search DNA sequences for sequence tagged sites (STSs), each of which is defined by a pair of primer sequences and an expected PCR product size. To gain speed, our implementation extracts short 'words' from the 3' end of each primer and stores them in a sorted hash table that can be accessed efficiently during the searc...
متن کاملA density based clustering approach to distinguish between web robot and human requests to a web server
Today world's dependence on the Internet and the emerging of Web 2.0 applications is significantly increasing the requirement of web robots crawling the sites to support services and technologies. Regardless of the advantages of robots, they may occupy the bandwidth and reduce the performance of web servers. Despite a variety of researches, there is no accurate method for classifying huge data ...
متن کاملPerforming Real-Time PCR
Optimisation of the reagents used to perform PCR is critical for reliable and reproducible results. As with any PCR initial time spent on optimisation of a real-time assay will be benefi cial in the long run. Specifi city, sensitivity, effi ciency and reproducibility are the important criteria to consider when optimising an assay and these can be altered by changes in the primer concentration, ...
متن کاملVizPrimer: a web server for visualized PCR primer design based on known gene structure
SUMMARY The visualization of gene structure plays an important role in polymerase chain reaction (PCR) primer design, especially for eukaryotic genes with a number of splice variants that users need to distinguish between via PCR. Here, we describe a visualized web server for primer design named VizPrimer. It utilizes the new information technology (IT) tools, HTML5 to display gene structure an...
متن کاملChromaligner: a web server for chromatogram alignment
UNLABELLED Chromaligner is a tool for chromatogram alignment to align retention time for chromatographic methods coupled to spectrophotometers such as high performance liquid chromatography and capillary electrophoresis for metabolomics works. Chromaligner resolves peak shifts by a constrained chromatogram alignment. For a collection of chromatograms and a set of defined peaks, Chromaligner ali...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 2004
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/gkh450